3WS5

N288Q-N321Q mutant BETA-LACTAMASE DERIVED FROM CHROMOHALOBACTER SP.560 (Condition-2B)

3WS5 の概要

• エントリーDOI: 10.2210/pdb3ws5/pdb
• 関連するPDBエントリー: 3WRT 3WRZ 3WS0 3WS1 3WS2 3WS4
• 分子名称: Beta-lactamase, STRONTIUM ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: cephalosporinase, hydrolase
• 由来する生物種: Chromohalobacter sp. 560
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 119022.35

構造登録者

Arai, S.,Yonezawa, Y.,Okazaki, N.,Matsumoto, F.,Shimizu, R.,Yamada, M.,Adachi, M.,Tamada, T.,Tokunaga, H.,Ishibashi, M.,Tokunaga, M.,Kuroki, R. (登録日: 2014-02-28, 公開日: 2015-03-04, 最終更新日: 2023-11-08)

主引用文献

Arai, S.,Yonezawa, Y.,Okazaki, N.,Matsumoto, F.,Shibazaki, C.,Shimizu, R.,Yamada, M.,Adachi, M.,Tamada, T.,Kawamoto, M.,Tokunaga, H.,Ishibashi, M.,Blaber, M.,Tokunaga, M.,Kuroki, R.
Structure of a highly acidic beta-lactamase from the moderate halophile Chromohalobacter sp. 560 and the discovery of a Cs(+)-selective binding site
Acta Crystallogr.,Sect.D, 71:541-554, 2015

PubMed Abstract: Environmentally friendly absorbents are needed for Sr(2+) and Cs(+), as the removal of the radioactive Sr(2+) and Cs(+) that has leaked from the Fukushima Nuclear Power Plant is one of the most important problems in Japan. Halophilic proteins are known to have many acidic residues on their surface that can provide specific binding sites for metal ions such as Cs(+) or Sr(2+). The crystal structure of a halophilic β-lactamase from Chromohalobacter sp. 560 (HaBLA) was determined to resolutions of between 1.8 and 2.9 Å in space group P31 using X-ray crystallography. Moreover, the locations of bound Sr(2+) and Cs(+) ions were identified by anomalous X-ray diffraction. The location of one Cs(+)-specific binding site was identified in HaBLA even in the presence of a ninefold molar excess of Na(+) (90 mM Na(+)/10 mM Cs(+)). From an activity assay using isothermal titration calorimetry, the bound Sr(2+) and Cs(+) ions do not significantly affect the enzymatic function of HaBLA. The observation of a selective and high-affinity Cs(+)-binding site provides important information that is useful for the design of artificial Cs(+)-binding sites that may be useful in the bioremediation of radioactive isotopes.

PubMed: 25760604
DOI: 10.1107/S1399004714027734

実験手法

X-RAY DIFFRACTION (2.8 Å)