3WQ8

Monomer structure of hyperthermophilic beta-glucosidase mutant forming a dodecameric structure in the crystal form

3WQ8 の概要

• エントリーDOI: 10.2210/pdb3wq8/pdb
• 関連するPDBエントリー: 3APG 3WDP
• 分子名称: Beta-glucosidase (2 entities in total)
• 機能のキーワード: tim barrel, hydrolase, sugar binding, hydrolysis
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 622339.97

構造登録者

Nakabayashi, M.,Kataoka, M.,Watanabe, M.,Ishikawa, K. (登録日: 2014-01-23, 公開日: 2014-07-09, 最終更新日: 2023-11-08)

主引用文献

Nakabayashi, M.,Kataoka, M.,Watanabe, M.,Ishikawa, K.
Monomer structure of a hyperthermophilic beta-glucosidase mutant forming a dodecameric structure in the crystal form.
Acta Crystallogr.,Sect.F, 70:854-859, 2014

PubMed Abstract: One of the β-glucosidases from Pyrococcus furiosus (BGLPf) is found to be a hyperthermophilic tetrameric enzyme that can degrade cellooligosaccharides. Recently, the crystal structures of the tetrameric and dimeric forms were solved. Here, a new monomeric form of BGLPf was constructed by removing the C-terminal region of the enzyme and its crystal structure was solved at a resolution of 2.8 Å in space group P1. It was discovered that the mutant enzyme forms a unique dodecameric structure consisting of two hexameric rings in the asymmetric unit of the crystal. Under biological conditions, the mutant enzyme forms a monomer. This result helps explain how BGLPf has attained its oligomeric structure and thermostability.

PubMed: 25005077
DOI: 10.1107/S2053230X14010188

実験手法

X-RAY DIFFRACTION (2.81 Å)