3WPX

Structure of PomBc4, a periplasmic fragment of PomB from Vibrio alginolyticus

3WPX の概要

• エントリーDOI: 10.2210/pdb3wpx/pdb
• 関連するPDBエントリー: 3WPW
• 分子名称: PomB (2 entities in total)
• 機能のキーワード: ompa-like, stator, peptidoglycan, membrane protein
• 由来する生物種: Vibrio alginolyticus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45693.01

構造登録者

Takao, M.,Sakuma, M.,Zhu, S.,Homma, M.,Kojima, S.,Imada, K. (登録日: 2014-01-17, 公開日: 2014-09-10, 最終更新日: 2023-11-08)

主引用文献

Zhu, S.,Takao, M.,Li, N.,Sakuma, M.,Nishino, Y.,Homma, M.,Kojima, S.,Imada, K.
Conformational change in the periplasmic region of the flagellar stator coupled with the assembly around the rotor
Proc. Natl. Acad. Sci. U.S.A., 111:13523-13528, 2014

PubMed Abstract: The torque of the bacterial flagellum is generated by the rotor-stator interaction coupled with the ion flow through the channel in the stator. Anchoring the stator unit to the peptidoglycan layer with proper orientation around the rotor is believed to be essential for smooth rotation of the flagellar motor. The stator unit of the sodium-driven flagellar motor of Vibrio is composed of PomA and PomB, and is thought to be fixed to the peptidoglycan layer and the T-ring by the C-terminal periplasmic region of PomB. Here, we report the crystal structure of a C-terminal fragment of PomB (PomBC) at 2.0-Å resolution, and the structure suggests a conformational change in the N-terminal region of PomBC for anchoring the stator. On the basis of the structure, we designed double-Cys replaced mutants of PomB for in vivo disulfide cross-linking experiments and examined their motility. The motility can be controlled reproducibly by reducing reagent. The results of these experiments suggest that the N-terminal disordered region (121-153) and following the N-terminal two-thirds of α1(154-164) in PomBC changes its conformation to form a functional stator around the rotor. The cross-linking did not affect the localization of the stator nor the ion conductivity, suggesting that the conformational change occurs in the final step of the stator assembly around the rotor.

PubMed: 25197056
DOI: 10.1073/pnas.1324201111

実験手法

X-RAY DIFFRACTION (2.3 Å)