3WPJ

SPATIOTEMPORAL DEVELOPMENT of SOAKED PROTEIN CRYSTAL; NATIVE

3WPJ の概要

• エントリーDOI: 10.2210/pdb3wpj/pdb
• 関連するPDBエントリー: 3wpk 3WPL 3wu7 3wu8 3wu9 3wua
• 分子名称: Lysozyme C (2 entities in total)
• 機能のキーワード: bacterial cell wall lysis, hydrolase
• 由来する生物種: Gallus gallus (bantam,chickens)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14331.16

構造登録者

Mizutani, R.,Saiga, R. (登録日: 2014-01-12, 公開日: 2014-07-30, 最終更新日: 2024-11-20)

主引用文献

Mizutani, R.,Shimizu, Y.,Saiga, R.,Ueno, G.,Nakamura, Y.,Takeuchi, A.,Uesugi, K.,Suzuki, Y.
Spatiotemporal development of soaked protein crystal
Sci Rep, 4:5731-5731, 2014

PubMed Abstract: Crystal soaking is widely performed in biological crystallography. This paper reports time-resolved X-ray crystallographic and microtomographic analyses of tetragonal crystals of chicken egg-white lysozyme soaked in mother liquor containing potassium hexachloroplatinate. The microtomographic analysis showed that X-ray attenuation spread from the superficial layer of the crystal and then to the crystal core. The crystallographic analyses indicated that platinum sites can be classified into two groups from the temporal development of the electron densities. A soaking process consisting of binding-rate-driven and equilibrium-driven layers is proposed to describe these results. This study suggests that the composition of chemical and structural species resulting from the soaking process varies depending on the position in the crystal.

PubMed: 25043871
DOI: 10.1038/srep05731

実験手法

X-RAY DIFFRACTION (2 Å)