3WMP

Crystal structure of SLL-2

3WMP の概要

• エントリーDOI: 10.2210/pdb3wmp/pdb
• 関連するPDBエントリー: 3WMQ
• 分子名称: Galactose-binding lectin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (8 entities in total)
• 機能のキーワード: six-stranded antiparallel-beta sandwich, galactose binding protein, sugar binding protein
• 由来する生物種: Sinularia lochmodes
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 68641.67

構造登録者

Kita, A.,Jimbo, M.,Sakai, R.,Morimoto, Y.,Miki, K. (登録日: 2013-11-22, 公開日: 2015-01-14, 最終更新日: 2024-11-20)

主引用文献

Kita, A.,Jimbo, M.,Sakai, R.,Morimoto, Y.,Miki, K.
Crystal structure of a symbiosis-related lectin from octocoral.
Glycobiology, 25:1016-1023, 2015

PubMed Abstract: D-Galactose-binding lectin from the octocoral, Sinularia lochmodes (SLL-2), distributes densely on the cell surface of microalgae, Symbiodinium sp., an endosymbiotic dinoflagellate of the coral, and is also shown to be a chemical cue that transforms dinoflagellate into a non-motile (coccoid) symbiotic state. SLL-2 binds with high affinity to the Forssman antigen (N-acetylgalactosamine(GalNAc)α1-3GalNAcβ1-3Galα1-4Galβ1-4Glc-ceramide), and the presence of Forssman antigen-like sugar on the surface of Symbiodinium CS-156 cells was previously confirmed. Here we report the crystal structures of SLL-2 and its GalNAc complex as the first crystal structures of a lectin involved in the symbiosis between coral and dinoflagellate. N-Linked sugar chains and a galactose derivative binding site common to H-type lectins were observed in each monomer of the hexameric SLL-2 crystal structure. In addition, unique sugar-binding site-like regions were identified at the top and bottom of the hexameric SLL-2 structure. These structural features suggest a possible binding mode between SLL-2 and Forssman antigen-like pentasaccharide.

PubMed: 26022515
DOI: 10.1093/glycob/cwv033

実験手法

X-RAY DIFFRACTION (2 Å)