3WLU

Crystal Structure of human galectin-9 NCRD with Selenolactose

Summary for 3WLU

• Entry DOI: 10.2210/pdb3wlu/pdb
• Related: 2EAK
• Descriptor: Galectin-9, 2-(trimethylsilyl)ethyl 4-O-beta-D-galactopyranosyl-6-Se-methyl-6-seleno-beta-D-glucopyranoside (3 entities in total)
• Functional Keywords: selenolactose, beta sandwich, galectin, carbohydrate binding, sugar binding protein
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm : O00182
• Total number of polymer chains: 4
• Total formula weight: 65765.38

Authors

Makyio, H.,Suzuki, T.,Ando, H.,Yamada, Y.,Ishida, H.,Kiso, M.,Wakatsuki, S.,Kato, R. (deposition date: 2013-11-14, release date: 2014-11-05, Last modification date: 2024-03-20)

Primary citation

Suzuki, T.,Makyio, H.,Ando, H.,Komura, N.,Menjo, M.,Yamada, Y.,Imamura, A.,Ishida, H.,Wakatsuki, S.,Kato, R.,Kiso, M.
Expanded potential of seleno-carbohydrates as a molecular tool for X-ray structural determination of a carbohydrate-protein complex with single/multi-wavelength anomalous dispersion phasing
Bioorg.Med.Chem., 22:2090-2101, 2014

PubMed Abstract: Seleno-lactoses have been successfully synthesized as candidates for mimicking carbohydrate ligands for human galectin-9 N-terminal carbohydrate recognition domain (NCRD). Selenium was introduced into the mono- or di-saccharides using p-methylselenobenzoic anhydride (Tol2Se) as a novel selenating reagent. The TolSe-substituted monosaccharides were converted into selenoglycosyl donors or acceptors, which were reacted with coupling partners to afford seleno-lactoses. The seleno-lactoses were converted to the target compounds. The structure of human galectin-9 NCRD co-crystallized with 6-MeSe-lactose was determined with single/multi-wavelength anomalous dispersion (SAD/MAD) phasing and was similar to that of the co-crystal with natural lactose.

PubMed: 24631362
DOI: 10.1016/j.bmc.2014.02.023

Experimental method

X-RAY DIFFRACTION (1.4 Å)