6MI1

CRYSTAL STRUCTURE ANALYSIS OF THE VARIANT PLANT EXOHYDROLASE ARG158ALA-GLU161ALA IN COMPLEX WITH METHYL 6-THIO-BETA-GENTIOBIOSIDE

Replaces:  3WLS

Summary for 6MI1

• Entry DOI: 10.2210/pdb6mi1/pdb
• Related: 1EX1 1IEQ 1IEV 1IEW 1IEX 1J8V 3WLH 3WLI 3WLJ 3WLK 3WLL 3WLM 3WLN 3WLO 3WLP 3WLQ 3WLR 3WLT
• Related PRD ID: PRD_900098
• Descriptor: Beta-D-glucan exohydrolase isoenzyme ExoI, beta-D-glucopyranose-(1-6)-methyl 6-thio-beta-D-glucopyranoside, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: beta barrel, hydrolase, grain development, tim barrel/beta sheet, n-glycosylation, plant apoplast
• Biological source: Hordeum vulgare subsp. vulgare (Domesticated barley)
• Total number of polymer chains: 1
• Total formula weight: 67995.06

Authors

Streltsov, V.A.,Luang, S.,Hrmova, M. (deposition date: 2018-09-18, release date: 2019-05-29, Last modification date: 2023-10-11)

• Primary citation: Streltsov, V.A.,Luang, S.,Peisley, A.,Varghese, J.N.,Ketudat Cairns, J.R.,Fort, S.,Hijnen, M.,Tvaroska, I.,Arda, A.,Jimenez-Barbero, J.,Alfonso-Prieto, M.,Rovira, C.,Mendoza, F.,Tiessler-Sala, L.,Sanchez-Aparicio, J.E.,Rodriguez-Guerra, J.,Lluch, J.M.,Marechal, J.D.,Masgrau, L.,Hrmova, M.; Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site.; Nat Commun, 10:2222-2222, 2019; PubMed: 31110237; DOI: 10.1038/s41467-019-09691-z

Experimental method

X-RAY DIFFRACTION (2.3 Å)