3WH2

Human Mincle in complex with citrate

3WH2 の概要

• エントリーDOI: 10.2210/pdb3wh2/pdb
• 関連するPDBエントリー: 3WH3
• 分子名称: C-type lectin domain family 4 member E, CALCIUM ION, CITRATE ANION, ... (4 entities in total)
• 機能のキーワード: c-type lectin, carbohydrate recognition, glycolipid binding, plasmamembrane, immune system
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Single-pass type II membrane protein (Probable): Q9ULY5
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17147.33

構造登録者

Furukawa, A.,Kamishikiryo, J.,Mori, D.,Toyonaga, K.,Okabe, Y.,Toji, A.,Kanda, R.,Miyake, Y.,Ose, T.,Yamasaki, S.,Maenaka, K. (登録日: 2013-08-21, 公開日: 2013-10-23, 最終更新日: 2024-11-13)

主引用文献

Furukawa, A.,Kamishikiryo, J.,Mori, D.,Toyonaga, K.,Okabe, Y.,Toji, A.,Kanda, R.,Miyake, Y.,Ose, T.,Yamasaki, S.,Maenaka, K.
Structural analysis for glycolipid recognition by the C-type lectins Mincle and MCL
Proc.Natl.Acad.Sci.USA, 110:17438-17443, 2013

PubMed Abstract: Mincle [macrophage inducible Ca(2+)-dependent (C-type) lectin; CLEC4E] and MCL (macrophage C-type lectin; CLEC4D) are receptors for the cord factor TDM (trehalose-6,6'-dimycolate), a unique glycolipid of mycobacterial cell-surface components, and activate immune cells to confer adjuvant activity. Although it is known that receptor-TDM interactions require both sugar and lipid moieties of TDM, the mechanisms of glycolipid recognition by Mincle and MCL remain unclear. We here report the crystal structures of Mincle, MCL, and the Mincle-citric acid complex. The structures revealed that these receptors are capable of interacting with sugar in a Ca(2+)-dependent manner, as observed in other C-type lectins. However, Mincle and MCL uniquely possess shallow hydrophobic regions found adjacent to their putative sugar binding sites, which reasonably locate for recognition of fatty acid moieties of glycolipids. Functional studies using mutant receptors as well as glycolipid ligands support this deduced binding mode. These results give insight into the molecular mechanism of glycolipid recognition through C-type lectin receptors, which may provide clues to rational design for effective adjuvants.

PubMed: 24101491
DOI: 10.1073/pnas.1312649110

実験手法

X-RAY DIFFRACTION (1.3 Å)