3WGX
Crystal structure of ERp46 Trx2 in a complex with Prx4 C-term
Summary for 3WGX
| Entry DOI | 10.2210/pdb3wgx/pdb |
| Descriptor | Thioredoxin domain-containing protein 5, Peroxiredoxin-4, GLYCEROL, ... (4 entities in total) |
| Functional Keywords | pdi family member, thioredoxin domain, protein disulfide isomerase, isomerase |
| Biological source | Homo sapiens (human) More |
| Cellular location | Endoplasmic reticulum lumen (By similarity): Q8NBS9 Cytoplasm (By similarity): O08807 |
| Total number of polymer chains | 4 |
| Total formula weight | 30227.96 |
| Authors | Inaba, K.,Suzuki, M.,Kojima, R. (deposition date: 2013-08-13, release date: 2014-06-25, Last modification date: 2024-10-30) |
| Primary citation | Kojima, R.,Okumura, M.,Masui, S.,Kanemura, S.,Inoue, M.,Saiki, M.,Yamaguchi, H.,Hikima, T.,Suzuki, M.,Akiyama, S.,Inaba, K. Radically different thioredoxin domain arrangement of ERp46, an efficient disulfide bond introducer of the mammalian PDI family Structure, 22:431-443, 2014 Cited by PubMed Abstract: The mammalian endoplasmic reticulum (ER) contains a diverse oxidative protein folding network in which ERp46, a member of the protein disulfide isomerase (PDI) family, serves as an efficient disulfide bond introducer together with Peroxiredoxin-4 (Prx4). We revealed a radically different molecular architecture of ERp46, in which the N-terminal two thioredoxin (Trx) domains with positively charged patches near their peptide-binding site and the C-terminal Trx are linked by unusually long loops and arranged extendedly, forming an opened V-shape. Whereas PDI catalyzes native disulfide bond formation by the cooperative action of two mutually facing redox-active sites on folding intermediates bound to the central cleft, ERp46 Trx domains are separated, act independently, and engage in rapid but promiscuous disulfide bond formation during early oxidative protein folding. Thus, multiple PDI family members likely contribute to different stages of oxidative folding and work cooperatively to ensure the efficient production of multi-disulfide proteins in the ER. PubMed: 24462249DOI: 10.1016/j.str.2013.12.013 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (0.92 Å) |
Structure validation
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