3WG7

A 1.9 angstrom radiation damage free X-ray structure of large (420KDa) protein by femtosecond crystallography

Summary for 3WG7

• Entry DOI: 10.2210/pdb3wg7/pdb
• Descriptor: Cytochrome c oxidase subunit 1, Cytochrome c oxidase subunit 7A1, mitochondrial, Cytochrome c oxidase subunit 7B, mitochondrial, ... (28 entities in total)
• Functional Keywords: electron transport complex iv, iron, oxidation-reduction, protein interaction domains, motifs, oxidoreductase, membrane
• Biological source: Bos taurus (bovine,cow,domestic cattle,domestic cow); More
• Cellular location: Mitochondrion inner membrane; Multi-pass membrane protein: P00396 P68530 P00415; Mitochondrion inner membrane: P07470 P13183 P00430 P10175 P00423 P00426 P00428 P07471 P04038; Mitochondrion intermembrane space: P00429
• Total number of polymer chains: 26
• Total formula weight: 441900.85

Authors

Hirata, K.,Shinzawa-Itoh, K.,Yano, N.,Takemura, S.,Kato, K.,Hatanaka, M.,Muramoto, K.,Kawahara, T.,Tsukihara, T.,Yamashita, E.,Tono, K.,Ueno, G.,Hikima, T.,Murakami, H.,Inubushi, Y.,Yabashi, M.,Ishikawa, T.,Yamamoto, M.,Ogura, T.,Sugimoto, H.,Shen, J.R.,Yoshikawa, S.,Ago, H. (deposition date: 2013-07-29, release date: 2014-04-30, Last modification date: 2023-11-08)

Primary citation

Hirata, K.,Shinzawa-Itoh, K.,Yano, N.,Takemura, S.,Kato, K.,Hatanaka, M.,Muramoto, K.,Kawahara, T.,Tsukihara, T.,Yamashita, E.,Tono, K.,Ueno, G.,Hikima, T.,Murakami, H.,Inubushi, Y.,Yabashi, M.,Ishikawa, T.,Yamamoto, M.,Ogura, T.,Sugimoto, H.,Shen, J.R.,Yoshikawa, S.,Ago, H.
Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL.
Nat.Methods, 11:734-736, 2014

PubMed Abstract: We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.

PubMed: 24813624
DOI: 10.1038/nmeth.2962

Experimental method

X-RAY DIFFRACTION (1.9 Å)