3WG2

Crystal structure of Agrocybe cylindracea galectin mutant (N46A)

Summary for 3WG2

• Entry DOI: 10.2210/pdb3wg2/pdb
• Related: 3WG1 3WG3 3WG4
• Descriptor: Galactoside-binding lectin, TRIETHYLENE GLYCOL (3 entities in total)
• Functional Keywords: galectin, galactose binding, sugar binding protein
• Biological source: Agrocybe aegerita
• Total number of polymer chains: 2
• Total formula weight: 38072.33

Authors

Kuwabara, N.,Hu, D.,Tateno, H.,Makio, H.,Hirabayashi, J.,Kato, R. (deposition date: 2013-07-25, release date: 2013-11-27, Last modification date: 2023-11-08)

Primary citation

Kuwabara, N.,Hu, D.,Tateno, H.,Makyio, H.,Hirabayashi, J.,Kato, R.
Conformational change of a unique sequence in a fungal galectin from Agrocybe cylindracea controls glycan ligand-binding specificity.
Febs Lett., 587:3620-3625, 2013

PubMed Abstract: A fungal galectin from Agrocybe cylindracea (ACG) exhibits broad binding specificity for β-galactose-containing glycans. We determined the crystal structures of wild-type ACG and the N46A mutant, with and without glycan ligands. From these structures and a saccharide-binding analysis of the N46A mutant, we revealed that a conformational change of a unique insertion sequence containing Asn46 provides two binding modes for ACG, and thereby confers broad binding specificity. We propose that the unique sequence provides these two distinct glycan-binding modes by an induced-fit mechanism.

PubMed: 24036446
DOI: 10.1016/j.febslet.2013.08.046

Experimental method

X-RAY DIFFRACTION (2.2 Å)