3WCQ
Crystal structure analysis of Cyanidioschyzon melorae ferredoxin D58N mutant
Summary for 3WCQ
| Entry DOI | 10.2210/pdb3wcq/pdb |
| Descriptor | Ferredoxin, FE2/S2 (INORGANIC) CLUSTER (3 entities in total) |
| Functional Keywords | 2fe-2s cluster, electron transfer, fnr, electron transport |
| Biological source | Cyanidioschyzon merolae (Red alga) |
| Total number of polymer chains | 1 |
| Total formula weight | 10755.62 |
| Authors | Ueno, Y.,Matsumoto, T.,Yamano, A.,Imai, T.,Morimoto, Y. (deposition date: 2013-05-31, release date: 2013-08-07, Last modification date: 2024-03-20) |
| Primary citation | Ueno, Y.,Matsumoto, T.,Yamano, A.,Imai, T.,Morimoto, Y. Increasing the electron-transfer ability of Cyanidioschyzon merolae ferredoxin by a one-point mutation - A high resolution and Fe-SAD phasing crystal structure analysis of the Asp58Asn mutant Biochem.Biophys.Res.Commun., 436:736-739, 2013 Cited by PubMed Abstract: Cyanidioschyzon merolae (Cm) is a single cell red algae that grows in rather thermophilic (40-50°C) and acidic (pH 1-3) conditions. Ferredoxin (Fd) was purified from this algae and characterized as a plant-type [2Fe-2S] Fd by physicochemical techniques. A high resolution (0.97Å) three-dimensional structure of the CmFd D58N mutant molecule has been determined using the Fe-SAD phasing method to clarify the precise position of the Asn58 amide, as this substitution increases the electron-transfer ability relative to wild-type CmFd by a factor of 1.5. The crystal structure reveals an electro-positive surface surrounding Asn58 that may interact with ferredoxin NADP(+) reductase or cytochrome c. PubMed: 23792094DOI: 10.1016/j.bbrc.2013.06.029 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (0.97 Å) |
Structure validation
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