3WCQ

Crystal structure analysis of Cyanidioschyzon melorae ferredoxin D58N mutant

3WCQ の概要

• エントリーDOI: 10.2210/pdb3wcq/pdb
• 分子名称: Ferredoxin, FE2/S2 (INORGANIC) CLUSTER (3 entities in total)
• 機能のキーワード: 2fe-2s cluster, electron transfer, fnr, electron transport
• 由来する生物種: Cyanidioschyzon merolae (Red alga)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10755.62

構造登録者

Ueno, Y.,Matsumoto, T.,Yamano, A.,Imai, T.,Morimoto, Y. (登録日: 2013-05-31, 公開日: 2013-08-07, 最終更新日: 2024-03-20)

主引用文献

Ueno, Y.,Matsumoto, T.,Yamano, A.,Imai, T.,Morimoto, Y.
Increasing the electron-transfer ability of Cyanidioschyzon merolae ferredoxin by a one-point mutation - A high resolution and Fe-SAD phasing crystal structure analysis of the Asp58Asn mutant
Biochem.Biophys.Res.Commun., 436:736-739, 2013

PubMed Abstract: Cyanidioschyzon merolae (Cm) is a single cell red algae that grows in rather thermophilic (40-50°C) and acidic (pH 1-3) conditions. Ferredoxin (Fd) was purified from this algae and characterized as a plant-type [2Fe-2S] Fd by physicochemical techniques. A high resolution (0.97Å) three-dimensional structure of the CmFd D58N mutant molecule has been determined using the Fe-SAD phasing method to clarify the precise position of the Asn58 amide, as this substitution increases the electron-transfer ability relative to wild-type CmFd by a factor of 1.5. The crystal structure reveals an electro-positive surface surrounding Asn58 that may interact with ferredoxin NADP(+) reductase or cytochrome c.

PubMed: 23792094
DOI: 10.1016/j.bbrc.2013.06.029

実験手法

X-RAY DIFFRACTION (0.97 Å)