3VWS

Dengue serotype 3 RNA-dependent RNA polymerase bound to NITD-107

3VWS の概要

• エントリーDOI: 10.2210/pdb3vws/pdb
• 分子名称: Non-structural protein 5, ZINC ION, 5-{[(4-chlorophenyl)sulfonyl]amino}-2-methyl-1-benzofuran-3-carboxylic acid, ... (5 entities in total)
• 機能のキーワード: rna-dependent rna polymerase, rna polymerase, viral protein, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• 由来する生物種: Dengue virus 3
• 細胞内の位置: Envelope protein E: Virion membrane; Multi- pass membrane protein: Q6DLV0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 74593.38

構造登録者

Noble, C.G.,Lescar, J. (登録日: 2012-08-31, 公開日: 2013-02-27, 最終更新日: 2023-11-08)

主引用文献

Noble, C.G.,Lim, S.P.,Chen, Y.L.,Liew, C.W.,Yap, L.,Lescar, J.,Shi, P.-Y.
Conformational flexibility of the Dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor
J.Virol., 87:5291-5295, 2013

PubMed Abstract: We report a highly reproducible method to crystallize the RNA-dependent RNA polymerase (RdRp) domain of dengue virus serotype 3 (DENV-3), allowing structure refinement to a 1.79-Å resolution and revealing amino acids not seen previously. We also present a DENV-3 polymerase/inhibitor cocrystal structure at a 2.1-Å resolution. The inhibitor binds to the RdRp as a dimer and causes conformational changes in the protein. The improved crystallization conditions and new structural information should accelerate structure-based drug discovery.

PubMed: 23408636
DOI: 10.1128/JVI.00045-13

実験手法

X-RAY DIFFRACTION (2.1 Å)