3VW4

Crystal structure of the DNA-binding domain of ColE2-P9 Rep in complex with the replication origin

Summary for 3VW4

• Entry DOI: 10.2210/pdb3vw4/pdb
• Descriptor: Rep, DNA (5'-D(P*AP*AP*TP*GP*AP*GP*AP*CP*CP*AP*GP*AP*TP*AP*AP*GP*CP*CP*TP*TP*AP*TP*C)-3'), DNA (5'-D(P*GP*AP*TP*AP*AP*GP*GP*CP*TP*TP*AP*TP*CP*TP*GP*GP*TP*CP*TP*CP*AP*TP*T)-3'), ... (5 entities in total)
• Functional Keywords: helix-turn-helix, specific dna-binding and unwinding of dna duplex, cytosol, replication initiator protein, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Escherichia coli; More
• Total number of polymer chains: 6
• Total formula weight: 58172.65

Authors

Itou, H.,Yagura, M.,Itoh, T.,Shirakihara, Y. (deposition date: 2012-07-31, release date: 2013-07-31, Last modification date: 2024-03-20)

• Primary citation: Itou, H.,Yagura, M.,Shirakihara, Y.,Itoh, T.; Structural Basis for Replication Origin Unwinding by An Initiator-Primase of Plasmid ColE2-P9: Duplex DNA Unwinding by A Single Protein; J.Biol.Chem., 290:3601-3611, 2015; PubMed: 25538245; DOI: 10.1074/jbc.M114.595645

Experimental method

X-RAY DIFFRACTION (2.7 Å)