3VU4

Crystal structure of Kluyvelomyces marxianus Hsv2

Summary for 3VU4

• Entry DOI: 10.2210/pdb3vu4/pdb
• Descriptor: KmHsv2, SULFATE ION (3 entities in total)
• Functional Keywords: beta-propeller fold, protein transport
• Biological source: Kluyveromyces marxianus
• Total number of polymer chains: 2
• Total formula weight: 83155.10

Authors

Watanabe, Y.,Noda, N.N. (deposition date: 2012-06-15, release date: 2012-07-04, Last modification date: 2024-03-20)

Primary citation

Watanabe, Y.,Kobayashi, T.,Yamamoto, H.,Hoshida, H.,Akada, R.,Inagaki, F.,Ohsumi, Y.,Noda, N.N.
Structure-based analyses reveal distinct binding sites for Atg2 and phosphoinositides in Atg18.
J.Biol.Chem., 287:31681-31690, 2012

PubMed Abstract: Autophagy is an intracellular degradation system by which cytoplasmic materials are enclosed by an autophagosome and delivered to a lysosome/vacuole. Atg18 plays a critical role in autophagosome formation as a complex with Atg2 and phosphatidylinositol 3-phosphate (PtdIns(3)P). However, little is known about the structure of Atg18 and its recognition mode of Atg2 or PtdIns(3)P. Here, we report the crystal structure of Kluyveromyces marxianus Hsv2, an Atg18 paralog, at 2.6 Å resolution. The structure reveals a seven-bladed β-propeller without circular permutation. Mutational analyses of Atg18 based on the K. marxianus Hsv2 structure suggested that Atg18 has two phosphoinositide-binding sites at blades 5 and 6, whereas the Atg2-binding region is located at blade 2. Point mutations in the loops of blade 2 specifically abrogated autophagy without affecting another Atg18 function, the regulation of vacuolar morphology at the vacuolar membrane. This architecture enables Atg18 to form a complex with Atg2 and PtdIns(3)P in parallel, thereby functioning in the formation of autophagosomes at autophagic membranes.

PubMed: 22851171
DOI: 10.1074/jbc.M112.397570

Experimental method

X-RAY DIFFRACTION (2.6 Å)