3VRF

The crystal structure of hemoglobin from woolly mammoth in the carbonmonoxy forms

3VRF の概要

• エントリーDOI: 10.2210/pdb3vrf/pdb
• 関連するPDBエントリー: 3VFE 3VFG
• 分子名称: Hemoglobin subunit alpha, Hemoglobin subunit beta/delta hybrid, PROTOPORPHYRIN IX CONTAINING FE, ... (5 entities in total)
• 機能のキーワード: woolly mammoth hemoglobin, oxygen transport, oxygen storage/transport
• 由来する生物種: Mammuthus primigenius (mammoth); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33008.01

構造登録者

Noguchi, H.,Campbell, K.L.,Ho, C.,Park, S.-Y.,Tame, J.R.H. (登録日: 2012-04-09, 公開日: 2012-11-07, 最終更新日: 2023-11-08)

主引用文献

Noguchi, H.,Campbell, K.L.,Ho, C.,Unzai, S.,Park, S.-Y.,Tame, J.R.H.
Structures of haemoglobin from woolly mammoth in liganded and unliganded states.
Acta Crystallogr.,Sect.D, 68:1441-1449, 2012

PubMed Abstract: The haemoglobin (Hb) of the extinct woolly mammoth has been recreated using recombinant genes expressed in Escherichia coli. The globin gene sequences were previously determined using DNA recovered from frozen cadavers. Although highly similar to the Hb of existing elephants, the woolly mammoth protein shows rather different responses to chloride ions and temperature. In particular, the heat of oxygenation is found to be much lower in mammoth Hb, which appears to be an adaptation to the harsh high-latitude climates of the Pleistocene Ice Ages and has been linked to heightened sensitivity of the mammoth protein to protons, chloride ions and organic phosphates relative to that of Asian elephants. To elucidate the structural basis for the altered homotropic and heterotropic effects, the crystal structures of mammoth Hb have been determined in the deoxy, carbonmonoxy and aquo-met forms. These models, which are the first structures of Hb from an extinct species, show many features reminiscent of human Hb, but underline how the delicate control of oxygen affinity relies on much more than simple overall quaternary-structure changes.

PubMed: 23090393
DOI: 10.1107/S0907444912029459

実験手法

X-RAY DIFFRACTION (1.55 Å)