3VR1

Crystal structure analysis of the translation factor RF3

3VR1 の概要

• エントリーDOI: 10.2210/pdb3vr1/pdb
• 関連するPDBエントリー: 3VQT
• 分子名称: Peptide chain release factor 3, GUANOSINE-5',3'-TETRAPHOSPHATE (2 entities in total)
• 機能のキーワード: translation, release factor, gtpase
• 由来する生物種: Desulfovibrio vulgaris
• 細胞内の位置: Cytoplasm (By similarity): B8DIL5
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 247895.95

構造登録者

Kihira, K.,Shomura, Y.,Shibata, N.,Kitamura, M.,Higuchi, Y. (登録日: 2012-04-03, 公開日: 2012-09-05, 最終更新日: 2023-11-08)

主引用文献

Kihira, K.,Shimizu, Y.,Shomura, Y.,Shibata, N.,Kitamura, M.,Nakagawa, A.,Ueda, T.,Ochi, K.,Higuchi, Y.
Crystal structure analysis of the translation factor RF3 (release factor 3)
Febs Lett., 586:3705-3709, 2012

PubMed Abstract: The bacterial translational GTPases release factor RF3 promotes translation termination by recycling RF1 or RF2. Here, we present the crystal structures of RF3 complexed with GDP and guanosine 3',5'-(bis)diphosphate (ppGpp) at resolutions of 1.8 and 3.0Å, respectively. ppGpp is involved in the so-called "stringent response" of bacteria. ppGpp binds at the same site as GDP, suggesting that GDP and ppGpp are two alternative physiologically relevant ligands of RF3. We also found that ppGpp decelerates the recycling of RF1 by RF3. These lines of evidence suggest that RF3 functions both as a cellular metabolic sensor and as a regulator.

PubMed: 22975312
DOI: 10.1016/j.febslet.2012.08.029

実験手法

X-RAY DIFFRACTION (3 Å)