3VQE

HIV-1 IN core domain in complex with [1-(4-fluorophenyl)-5-methyl-1H-pyrazol-4-yl]methanol

Summary for 3VQE

• Entry DOI: 10.2210/pdb3vqe/pdb
• Related: 3VQ4 3VQ5 3VQ6 3VQ7 3VQ8 3VQ9 3VQA 3VQB 3VQC 3VQD 3VQP 3VQQ 3l3u 3l3v 3ovn
• Descriptor: POL polyprotein, CADMIUM ION, CHLORIDE ION, ... (6 entities in total)
• Functional Keywords: rnaseh, dna binding, dna cleavage, dna integration, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• Biological source: Human immunodeficiency virus 1
• Total number of polymer chains: 2
• Total formula weight: 35520.02

Authors

Wielens, J.,Chalmers, D.K.,Parker, M.W.,Scanlon, M.J. (deposition date: 2012-03-21, release date: 2013-01-30, Last modification date: 2023-11-08)

Primary citation

Wielens, J.,Headey, S.J.,Rhodes, D.I.,Mulder, R.J.,Dolezal, O.,Deadman, J.J.,Newman, J.,Chalmers, D.K.,Parker, M.W.,Peat, T.S.,Scanlon, M.J.
Parallel screening of low molecular weight fragment libraries: do differences in methodology affect hit identification?
J Biomol Screen, 18:147-159, 2013

PubMed Abstract: Fragment screening is becoming widely accepted as a technique to identify hit compounds for the development of novel lead compounds. In neighboring laboratories, we have recently, and independently, performed a fragment screening campaign on the HIV-1 integrase core domain (IN) using similar commercially purchased fragment libraries. The two campaigns used different screening methods for the preliminary identification of fragment hits; one used saturation transfer difference nuclear magnetic resonance spectroscopy (STD-NMR), and the other used surface plasmon resonance (SPR) spectroscopy. Both initial screens were followed by X-ray crystallography. Using the STD-NMR/X-ray approach, 15 IN/fragment complexes were identified, whereas the SPR/X-ray approach found 6 complexes. In this article, we compare the approaches that were taken by each group and the results obtained, and we look at what factors could potentially influence the final results. We find that despite using different approaches with little overlap of initial hits, both approaches identified binding sites on IN that provided a basis for fragment-based lead discovery and further lead development. Comparison of hits identified in the two studies highlights a key role for both the conditions under which fragment binding is measured and the criteria selected to classify hits.

PubMed: 23139382
DOI: 10.1177/1087057112465979

Experimental method

X-RAY DIFFRACTION (1.7 Å)