3VPR

Crystal Structure of a TetR Family Transcriptional Regulator PfmR from Thermus thermophilus HB8

3VPR の概要

• エントリーDOI: 10.2210/pdb3vpr/pdb
• 分子名称: Transcriptional regulator, TetR family (2 entities in total)
• 機能のキーワード: all alpha, helix-turn-helix, transcriptional repressor, dna binding protein
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 87473.44

構造登録者

Agari, Y.,Sakamoto, K.,Kuramitsu, S.,Shinkai, A. (登録日: 2012-03-12, 公開日: 2012-07-11, 最終更新日: 2024-11-13)

主引用文献

Agari, Y.,Sakamoto, K.,Kuramitsu, S.,Shinkai, A.
Transcriptional repression mediated by a TetR family protein, PfmR, from Thermus thermophilus HB8
J.Bacteriol., 2012

PubMed Abstract: PfmR is one of four TetR family transcriptional regulators found in the extremely thermophilic bacterium, Thermus thermophilus HB8. We identified three promoters with strong negative regulation by PfmR, both in vivo and in vitro. PfmR binds pseudopalindromic sequences, with the consensus sequence of 5'-TACCGACCGNTNGGTN-3' surrounding the promoters. According to the amino acid sequence and three-dimensional structure analyses of the PfmR-regulated gene products, they are predicted to be involved in phenylacetic acid and fatty acid metabolism. In vitro analyses revealed that PfmR weakly cross-regulated with the TetR family repressor T. thermophilus PaaR, which controls the expression of the paa gene cluster putatively involved in phenylacetic acid degradation but not with another functionally identified TetR family repressor, T. thermophilus FadR, which is involved in fatty acid degradation. The X-ray crystal structure of the N-terminal DNA-binding domain of PfmR and the nucleotide sequence of the predicted PfmR-binding site are quite similar to those of the TetR family repressor QacR from Staphylococcus aureus. Similar to QacR, two PfmR dimers bound per target DNA. The bases recognized by QacR within the QacR-binding site are conserved in the predicted PfmR-binding site, and they were important for PfmR to recognize the binding site and properly assemble on it. The center of the PfmR molecule contains a tunnel-like pocket, which may be the ligand-binding site of this regulator.

PubMed: 22753056
DOI: 10.1128/JB.00668-12

実験手法

X-RAY DIFFRACTION (2.27 Å)