3VNA

Structural insights into small RNA sorting and mRNA binding by Arabidopsis Ago Mid domains

3VNA の概要

• エントリーDOI: 10.2210/pdb3vna/pdb
• 関連するPDBエントリー: 3VNB
• 分子名称: Protein argonaute 1, SULFATE ION (3 entities in total)
• 機能のキーワード: rossmann fold, 5'-nucleotide small rna binding, rna binding protein
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• 細胞内の位置: Cytoplasm (Potential): O04379
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17891.60

構造登録者

Yuan, Y.A.,Zha, X. (登録日: 2012-01-11, 公開日: 2012-10-10, 最終更新日: 2024-03-20)

主引用文献

Zha, X.,Xia, Q.,Yuan, Y.A.
Structural insights into small RNA sorting and mRNA target binding by Arabidopsis Argonaute Mid domains
Febs Lett., 586:3200-3207, 2012

PubMed Abstract: The RISC-associated Argonaute (Ago) proteins play the catalytic role for RISC-mediated gene regulation by selecting small RNAs and subsequent targeting and cleavage of complementary mRNAs. Ago Mid domains are proposed to play essential roles in small RNA sorting. Here, we report the crystal structures of Arabidopsis Ago1 Mid domain and its chimera mutant with part of Ago1 replaced by Ago4. The structures demonstrate that a single amino insertion in the nucleotide specificity loop of AtAgo1 will change the nucleotide binding preference of AtAgo1 from "5'-U" to "5'-A". Moreover, we identify a long positively charged groove located along the "5'-end-nucleotide specificity loop" and occupied by several sulfate ions with the distance of 9-11Å distance, indicating a putative mRNA target binding groove.

PubMed: 22771475
DOI: 10.1016/j.febslet.2012.06.038

実験手法

X-RAY DIFFRACTION (2 Å)