3VIE

HIV-gp41 fusion inhibitor Sifuvirtide

3VIE の概要

• エントリーDOI: 10.2210/pdb3vie/pdb
• 分子名称: Envelope glycoprotein gp160, Sifuvirtide (3 entities in total)
• 機能のキーワード: 6-helix bundle, membrane fusion inhibition, hiv, viral protein-antiviral protein complex, viral protein/antiviral protein
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 26609.59

構造登録者

Yao, X.,Waltersperger, S.,Wang, M.T.,Cui, S. (登録日: 2011-09-29, 公開日: 2012-01-18, 最終更新日: 2023-11-08)

主引用文献

Yao, X.,Chong, H.,Zhang, C.,Waltersperger, S.,Wang, M.,Cui, S.,He, Y.
Broad antiviral activity and crystal structure of HIV-1 fusion inhibitor sifuvirtide
J.Biol.Chem., 287:6788-6796, 2012

PubMed Abstract: Sifuvirtide (SFT) is an electrostatically constrained α-helical peptide fusion inhibitor showing potent anti-HIV activity, good safety, and pharmacokinetic profiles, and it is currently under phase II clinical trials in China. In this study, we demonstrate its potent and broad anti-HIV activity by using diverse HIV-1 subtypes and variants, including subtypes A, B, and C that dominate the AIDS epidemic worldwide, and subtypes B', CRF07_BC, and CRF01_AE recombinants that are currently circulating in China, and those possessing cross-resistance to the first and second generation fusion inhibitors. To elucidate its mechanism of action, we determined the crystal structure of SFT in complex with its target N-terminal heptad repeat region (NHR) peptide (N36), which fully supports our rational inhibitor design and reveals its key motifs and residues responsible for the stability and anti-HIV activity. As anticipated, SFT adopts fully helical conformation stabilized by the multiple engineered salt bridges. The designing of SFT also provide novel inter-helical salt bridges and hydrogen bonds that improve the affinity of SFT to NHR trimer. The extra serine residue and acetyl group stabilize α-helicity of the N-terminal portion of SFT, whereas Thr-119 serves to stabilize the hydrophobic NHR pocket. In addition, our structure demonstrates that the residues critical for drug resistance, located at positions 37, 38, 41, and 43 of NHR, are irreplaceable for maintaining the stable fusogenic six-helix bundle structure. Our data present important information for developing SFT for clinical use and for designing novel HIV fusion inhibitors.

PubMed: 22228771
DOI: 10.1074/jbc.M111.317883

実験手法

X-RAY DIFFRACTION (1.8 Å)