3VEP

Crystal structure of SigD4 in complex with its negative regulator RsdA

Summary for 3VEP

• Entry DOI: 10.2210/pdb3vep/pdb
• Related: 3VFZ
• Descriptor: Uncharacterized protein Rv3413c/MT3522, Probable RNA polymerase sigma-D factor, SULFATE ION, ... (4 entities in total)
• Functional Keywords: sigma factor, promoter dna, anti-sigma factor, membrane protein-transcription complex, membrane protein/transcription
• Biological source: Mycobacterium tuberculosis; More
• Cellular location: Cell membrane; Single-pass membrane protein (Potential): P65081
• Total number of polymer chains: 8
• Total formula weight: 74816.89

Authors

Jaiswal, R.K.,Gopal, B. (deposition date: 2012-01-09, release date: 2013-02-13, Last modification date: 2024-10-09)

Primary citation

Jaiswal, R.K.,Prabha, T.S.,Manjeera, G.,Gopal, B.
Mycobacterium tuberculosis RsdA provides a conformational rationale for selective regulation of sigma-factor activity by proteolysis
Nucleic Acids Res., 41:3414-3423, 2013

PubMed Abstract: The relative levels of different σ factors dictate the expression profile of a bacterium. Extracytoplasmic function σ factors synchronize the transcriptional profile with environmental conditions. The cellular concentration of free extracytoplasmic function σ factors is regulated by the localization of this protein in a σ/anti-σ complex. Anti-σ factors are multi-domain proteins with a receptor to sense environmental stimuli and a conserved anti-σ domain (ASD) that binds a σ factor. Here we describe the structure of Mycobacterium tuberculosis anti-σ(D) (RsdA) in complex with the -35 promoter binding domain of σ(D) (σ(D)4). We note distinct conformational features that enable the release of σ(D) by the selective proteolysis of the ASD in RsdA. The structural and biochemical features of the σ(D)/RsdA complex provide a basis to reconcile diverse regulatory mechanisms that govern σ/anti-σ interactions despite high overall structural similarity. Multiple regulatory mechanisms embedded in an ASD scaffold thus provide an elegant route to rapidly re-engineer the expression profile of a bacterium in response to an environmental stimulus.

PubMed: 23314154
DOI: 10.1093/nar/gks1468

Experimental method

X-RAY DIFFRACTION (2.5 Å)