3VD7

E. coli (lacZ) beta-galactosidase (N460S) in complex with galactotetrazole

3VD7 の概要

• エントリーDOI: 10.2210/pdb3vd7/pdb
• 関連するPDBエントリー: 1DP0 1JZ6 3VD3 3VD4 3VD5 3VD9 3VDA 3VDB 3VDC
• 分子名称: Beta-galactosidase, (5R, 6S, 7S, 8S)-5-HYDROXYMETHYL-6,7,8-TRIHYDROXY-TETRAZOLO[1,5-A]PIPERIDINE, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: transition state stabilization, substrate binding, 2-stage binding, tim barrel (alpha/beta barrel), jelly-roll barrel, immunoglobulin, beta supersandwich, glycosidase, hydrolase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 482271.19

構造登録者

Wheatley, R.W.,Kappelhoff, J.C.,Hahn, J.N.,Dugdale, M.L.,Dutkoski, M.J.,Tamman, S.D.,Fraser, M.E.,Huber, R.E. (登録日: 2012-01-04, 公開日: 2012-04-11, 最終更新日: 2023-09-13)

• 主引用文献: Wheatley, R.W.,Kappelhoff, J.C.,Hahn, J.N.,Dugdale, M.L.,Dutkoski, M.J.,Tamman, S.D.,Fraser, M.E.,Huber, R.E.; Substitution for Asn460 cripples {beta}-galactosidase (Escherichia coli) by increasing substrate affinity and decreasing transition state stability.; Arch.Biochem.Biophys., 521:51-61, 2012; PubMed: 22446164; DOI: 10.1016/j.abb.2012.03.014

実験手法

X-RAY DIFFRACTION (2.87 Å)