3VCL

Crystal Structure of HLA-B7 with the HCMV pp65 peptide RPHERNGFTVL

3VCL の概要

• エントリーDOI: 10.2210/pdb3vcl/pdb
• 分子名称: HLA class I histocompatibility antigen, B-7 alpha chain, Beta-2-microglobulin, peptide from Tegument protein pp65, ... (6 entities in total)
• 機能のキーワード: class i histocompatibility antigen, antigen presentation, t-cell receptor, membrane, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P01889; Secreted: P61769
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 46010.20

構造登録者

Petersen, J.,Rossjohn, J. (登録日: 2012-01-04, 公開日: 2012-11-21, 最終更新日: 2024-11-20)

主引用文献

Brennan, R.M.,Petersen, J.,Neller, M.A.,Miles, J.J.,Burrows, J.M.,Smith, C.,McCluskey, J.,Khanna, R.,Rossjohn, J.,Burrows, S.R.
The impact of a large and frequent deletion in the human TCR beta locus on antiviral immunity
J.Immunol., 188:2742-2748, 2012

PubMed Abstract: The TCR plays a critical role in recognizing intracellular pathogens and initiating pathways leading to the destruction of infected cells by the immune system. Although genetic variability is known to greatly impact on the human immune system and the outcome of infection, the influence of sequence variation leading to the inactivation or deletion of TCR gene segments is unknown. To investigate this issue, we examined the CD8(+) T cell response to an HLA-B7-restricted epitope ((265)RPHERNGFTVL(275)) from the pp65 Ag of human CMV that was highly biased and frequently dominated by a public TCR β-chain encoded by the variable gene segment TRBV4-3. Approximately 40% of humans lack T cells expressing TRBV4-3 because of a 21.5-kb insertion/deletion polymorphism, but these individuals remain responsive to this epitope, using a diverse T cell repertoire characterized by private TCR usage. Although most residues within the bulged 11-mer peptide were accessible for TCR contact, the public and private TCRs showed distinct patterns of sensitivity to amino acid substitution at different positions within the peptide, thereby suggesting that the repertoire diversity generated in the absence of the dominant public TRBV4-3(+) TCR could lead to better protection from viral escape mutation. Thus, variation in the size of the TRBV repertoire clearly contributes toward interindividual variability in immune responses and is presumably maintained in many ethnic groups to enhance the diversity of Ag-specific T cell responses.

PubMed: 22323539
DOI: 10.4049/jimmunol.1102675

実験手法

X-RAY DIFFRACTION (1.7 Å)