3V6T

Crystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstrom

3V6T の概要

• エントリーDOI: 10.2210/pdb3v6t/pdb
• 関連するPDBエントリー: 3V6P
• 分子名称: dHax3, DNA (5'-D(*TP*GP*TP*CP*CP*CP*TP*TP*TP*AP*TP*CP*TP*CP*TP*CP*T)-3'), DNA (5'-D(*AP*GP*AP*GP*AP*GP*AP*TP*AP*AP*AP*GP*GP*GP*AP*CP*A)-3'), ... (4 entities in total)
• 機能のキーワード: 11 tandem repeats, dna specific binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Xanthomonas
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 124298.53

構造登録者

Deng, D.,Yan, C.Y.,Pan, X.J.,Wang, J.W.,Yan, N.,Shi, Y.G. (登録日: 2011-12-20, 公開日: 2012-01-18, 最終更新日: 2023-11-08)

主引用文献

Deng, D.,Yan, C.Y.,Pan, X.J.,Mahfouz, M.,Wang, J.W.,Zhu, J.K.,Shi, Y.G.,Yan, N.
Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors
Science, 2012

PubMed Abstract: TAL (transcription activator-like) effectors, secreted by phytopathogenic bacteria, recognize host DNA sequences through a central domain of tandem repeats. Each repeat comprises 33 to 35 conserved amino acids and targets a specific base pair by using two hypervariable residues [known as repeat variable diresidues (RVDs)] at positions 12 and 13. Here, we report the crystal structures of an 11.5-repeat TAL effector in both DNA-free and DNA-bound states. Each TAL repeat comprises two helices connected by a short RVD-containing loop. The 11.5 repeats form a right-handed, superhelical structure that tracks along the sense strand of DNA duplex, with RVDs contacting the major groove. The 12th residue stabilizes the RVD loop, whereas the 13th residue makes a base-specific contact. Understanding DNA recognition by TAL effectors may facilitate rational design of DNA-binding proteins with biotechnological applications.

PubMed: 22223738
DOI: 10.1126/science.1215670

実験手法

X-RAY DIFFRACTION (1.85 Å)