3V5R

Crystal structure of the unliganded form of Gal3p

Summary for 3V5R

• Entry DOI: 10.2210/pdb3v5r/pdb
• Related: 2AJ4 3V2U
• Descriptor: Protein GAL3, SULFATE ION (3 entities in total)
• Functional Keywords: ghmp superfamily, transcription transducer, gal80p, transcription
• Biological source: Saccharomyces cerevisiae (Baker's yeast)
• Total number of polymer chains: 2
• Total formula weight: 115623.54

Authors

Lavy, T.,Kumar, P.R.,He, H.,Joshua-Tor, L. (deposition date: 2011-12-16, release date: 2012-02-08, Last modification date: 2023-09-13)

Primary citation

Lavy, T.,Kumar, P.R.,He, H.,Joshua-Tor, L.
The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.
Genes Dev., 26:294-303, 2012

PubMed Abstract: A wealth of genetic information and some biochemical analysis have made the GAL regulon of the yeast Saccharomyces cerevisiae a classic model system for studying transcriptional activation in eukaryotes. Galactose induces this transcriptional switch, which is regulated by three proteins: the transcriptional activator Gal4p, bound to DNA; the repressor Gal80p; and the transducer Gal3p. We showed previously that NADP appears to act as a trigger to kick the repressor off the activator. Sustained activation involves a complex of the transducer Gal3p and Gal80p mediated by galactose and ATP. We solved the crystal structure of the complex of Gal3p-Gal80p with α-D-galactose and ATP to 2.1 Å resolution. The interaction between the proteins occurs only when Gal3p is in a "closed" state induced by ligand binding. The structure of the complex provides a rationale for the phenotypes of several well-known Gal80p and Gal3p mutants as well as the lack of galactokinase activity of Gal3p.

PubMed: 22302941
DOI: 10.1101/gad.182691.111

Experimental method

X-RAY DIFFRACTION (2.099 Å)