3V4D

Crystal structure of RutC protein a member of the YjgF family from E.coli

3V4D の概要

• エントリーDOI: 10.2210/pdb3v4d/pdb
• 分子名称: Aminoacrylate peracid reductase RutC (2 entities in total)
• 機能のキーワード: structural genomics, psi-biology, new york structural genomics research consortium, nysgrc, oxidoreductase
• 由来する生物種: Escherichia coli O6
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 87698.54

構造登録者

Knapik, A.A.,Petkowski, J.J.,Otwinowski, Z.,Cymborowski, M.T.,Cooper, D.R.,Chruszcz, M.,Porebski, P.J.,Niedzialkowska, E.,Almo, S.C.,Minor, W.,New York Structural Genomics Research Consortium (NYSGRC) (登録日: 2011-12-14, 公開日: 2012-01-04, 最終更新日: 2024-10-09)

主引用文献

Knapik, A.A.,Petkowski, J.J.,Otwinowski, Z.,Cymborowski, M.T.,Cooper, D.R.,Chruszcz, M.,Krajewska, W.M.,Minor, W.
Structure of Escherichia coli RutC, a member of the YjgF family and putative aminoacrylate peracid reductase of the rut operon.
Acta Crystallogr.,Sect.F, 68:1294-1299, 2012

PubMed Abstract: RutC is the third enzyme in the Escherichia coli rut pathway of uracil degradation. RutC belongs to the highly conserved YjgF family of proteins. The structure of the RutC protein was determined and refined to 1.95 Å resolution. The crystal belonged to space group P2(1)2(1)2 and contained six molecules in the asymmetric unit. The structure was solved by SAD phasing and was refined to an Rwork of 19.3% (Rfree=21.7%). The final model revealed that this protein has a Bacillus chorismate mutase-like fold and forms a homotrimer with a hydrophobic cavity in the center of the structure and ligand-binding clefts between two subunits. A likely function for RutC is the reduction of peroxy-aminoacrylate to aminoacrylate as a part of a detoxification process.

PubMed: 23143235
DOI: 10.1107/S1744309112041796

実験手法

X-RAY DIFFRACTION (1.95 Å)