3V3T

Crystal structure of Clostridium botulinum phage c-st TubZ

3V3T の概要

• エントリーDOI: 10.2210/pdb3v3t/pdb
• 分子名称: Cell division GTPase FtsZ, diverged (2 entities in total)
• 機能のキーワード: tubz, tubulin/ftsz related, rossmann fold, gtpase, gtp binding, structural protein
• 由来する生物種: Clostridium botulinum C
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40464.11

構造登録者

Oliva, M.A. (登録日: 2011-12-14, 公開日: 2012-05-16, 最終更新日: 2024-02-28)

主引用文献

Oliva, M.A.,Martin-Galiano, A.J.,Sakaguchi, Y.,Andreu, J.M.
Tubulin homolog TubZ in a phage-encoded partition system.
Proc.Natl.Acad.Sci.USA, 109:7711-7716, 2012

PubMed Abstract: Partition systems are responsible for the process whereby large and essential plasmids are accurately positioned to daughter cells during bacterial division. They are typically made of three components: a centromere-like DNA zone, an adaptor protein, and an assembling protein that is either a Walker-box ATPase (type I) or an actin-like ATPase (type II). A recently described type III segregation system has a tubulin/FtsZ-like protein, called TubZ, for plasmid movement. Here, we present the 2.3 Å structure and dynamic assembly of a TubZ tubulin homolog from a bacteriophage and unravel the Clostridium botulinum phage c-st type III partition system. Using biochemical and biophysical approaches, we prove that a gene upstream from tubZ encodes the partner TubR and localize the centromeric region (tubS), both of which are essential for anchoring phage DNA to the motile TubZ filaments. Finally, we describe a conserved fourth component, TubY, which modulates the TubZ-R-S complex interaction.

PubMed: 22538818
DOI: 10.1073/pnas.1121546109

実験手法

X-RAY DIFFRACTION (2.302 Å)