3UXW

Crystal Structures of an A-T-hook/DNA complex

3UXW の概要

• エントリーDOI: 10.2210/pdb3uxw/pdb
• 分子名称: dodecamer DNA, A-T hook peptide (3 entities in total)
• 機能のキーワード: a-t hook-dna complex, oligonucletide duplex hmga-1a protein, dna-peptide complex, dna/peptide
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 33797.03

構造登録者

Fonfria-Subiros, E.,Acosta-Reyes, F.J.,Saperas, N.,Pous, J.,Subirana, J.A.,Campos, J.L. (登録日: 2011-12-05, 公開日: 2012-05-23, 最終更新日: 2024-11-06)

主引用文献

Fonfria-Subiros, E.,Acosta-Reyes, F.,Saperas, N.,Pous, J.,Subirana, J.A.,Campos, J.L.
Crystal structure of a complex of DNA with one AT-hook of HMGA1.
Plos One, 7:e37120-e37120, 2012

PubMed Abstract: We present here for the first time the crystal structure of an AT-hook domain. We show the structure of an AT-hook of the ubiquitous nuclear protein HMGA1, combined with the oligonucleotide d(CGAATTAATTCG)(2), which has two potential AATT interacting groups. Interaction with only one of them is found. The structure presents analogies and significant differences with previous NMR studies: the AT-hook forms hydrogen bonds between main-chain NH groups and thymines in the minor groove, DNA is bent and the minor groove is widened.

PubMed: 22615915
DOI: 10.1371/journal.pone.0037120

実験手法

X-RAY DIFFRACTION (2.27 Å)