3UWX

Crystal structure of UvrA-UvrB complex

3UWX の概要

• エントリーDOI: 10.2210/pdb3uwx/pdb
• 分子名称: Excinuclease ABC, A subunit, UvrABC system protein B, ZINC ION (3 entities in total)
• 機能のキーワード: uvra, uvrb, nucleotide excision repair, dna repair, abc atpase, atp binding, dna binding, dna binding protein
• 由来する生物種: Geobacillus; 詳細
• 細胞内の位置: Cytoplasm (By similarity): E8SW61 E8SW62
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 186455.69

構造登録者

Pakotiprapha, D.,Jeruzalmi, D. (登録日: 2011-12-03, 公開日: 2012-02-08, 最終更新日: 2024-02-28)

主引用文献

Pakotiprapha, D.,Samuels, M.,Shen, K.,Hu, J.H.,Jeruzalmi, D.
Structure and mechanism of the UvrA-UvrB DNA damage sensor.
Nat.Struct.Mol.Biol., 19:291-298, 2012

PubMed Abstract: Nucleotide excision repair (NER) is used by all organisms to eliminate DNA lesions. We determined the structure of the Geobacillus stearothermophilus UvrA-UvrB complex, the damage-sensor in bacterial NER and a new structure of UvrA. We observe that the DNA binding surface of UvrA, previously found in an open shape that binds damaged DNA, also exists in a closed groove shape compatible with native DNA only. The sensor contains two UvrB molecules that flank the UvrA dimer along the predicted path for DNA, ~80 Å from the lesion. We show that the conserved signature domain II of UvrA mediates a nexus of contacts among UvrA, UvrB and DNA. Further, in our new structure of UvrA, this domain adopts an altered conformation while an adjacent nucleotide binding site is vacant. Our findings raise unanticipated questions about NER and also suggest a revised picture of its early stages.

PubMed: 22307053
DOI: 10.1038/nsmb.2240

実験手法

X-RAY DIFFRACTION (4.398 Å)