3UVF

Expanding LAGALIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence space

3UVF の概要

• エントリーDOI: 10.2210/pdb3uvf/pdb
• 関連するPDBエントリー: 1P8K 2QOJ 3EHB
• 分子名称: Intron-encoded DNA endonuclease I-HjeMI, synthetic oligo, CALCIUM ION, ... (8 entities in total)
• 機能のキーワード: laglidagd endonuclease, hydrolase, divalent metal ions, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Trichoderma reesei; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 94456.86

構造登録者

Jacoby, K.,Metzger, M.,Shen, B.,Jarjour, J.,Stoddard, B.,Scharenberg, A. (登録日: 2011-11-29, 公開日: 2012-02-29, 最終更新日: 2023-09-13)

主引用文献

Jacoby, K.,Metzger, M.,Shen, B.W.,Certo, M.T.,Jarjour, J.,Stoddard, B.L.,Scharenberg, A.M.
Expanding LAGLIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence space.
Nucleic Acids Res., 40:4954-4964, 2012

PubMed Abstract: LAGLIDADG homing endonucleases (LHEs) are a family of highly specific DNA endonucleases capable of recognizing target sequences ≈ 20 bp in length, thus drawing intense interest for their potential academic, biotechnological and clinical applications. Methods for rational design of LHEs to cleave desired target sites are presently limited by a small number of high-quality native LHEs to serve as scaffolds for protein engineering-many are unsatisfactory for gene targeting applications. One strategy to address such limitations is to identify close homologs of existing LHEs possessing superior biophysical or catalytic properties. To test this concept, we searched public sequence databases to identify putative LHE open reading frames homologous to the LHE I-AniI and used a DNA binding and cleavage assay using yeast surface display to rapidly survey a subset of the predicted proteins. These proteins exhibited a range of capacities for surface expression and also displayed locally altered binding and cleavage specificities with a range of in vivo cleavage activities. Of these enzymes, I-HjeMI demonstrated the greatest activity in vivo and was readily crystallizable, allowing a comparative structural analysis. Taken together, our results suggest that even highly homologous LHEs offer a readily accessible resource of related scaffolds that display diverse biochemical properties for biotechnological applications.

PubMed: 22334611
DOI: 10.1093/nar/gkr1303

実験手法

X-RAY DIFFRACTION (3 Å)