3UTB

Crystal Structure of Nucleosome Core Particle Assembled with the 146b Alpha-Satellite Sequence (NCP146b)

3UTB の概要

• エントリーDOI: 10.2210/pdb3utb/pdb
• 分子名称: Histone H3.2, Histone H4, Histone H2A, ... (8 entities in total)
• 機能のキーワード: nucleosome core particle, ncp, 146b dna, structural protein-dna complex, structural protein/dna
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• 細胞内の位置: Nucleus: P84233 P62799 P02281; Nucleus (By similarity): Q6AZJ8
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 200338.57

構造登録者

Chua, E.Y.D.,Vasudevan, D.,Davey, G.E.,Wu, B.,Davey, C.A. (登録日: 2011-11-25, 公開日: 2012-04-11, 最終更新日: 2024-03-20)

主引用文献

Chua, E.Y.D.,Vasudevan, D.,Davey, G.E.,Wu, B.,Davey, C.A.
The mechanics behind DNA sequence-dependent properties of the nucleosome
Nucleic Acids Res., 40:6338-6352, 2012

PubMed Abstract: Chromatin organization and composition impart sophisticated regulatory features critical to eukaryotic genomic function. Although DNA sequence-dependent histone octamer binding is important for nucleosome activity, many aspects of this phenomenon have remained elusive. We studied nucleosome structure and stability with diverse DNA sequences, including Widom 601 derivatives with the highest known octamer affinities, to establish a simple model behind the mechanics of sequence dependency. This uncovers the unique but unexpected role of TA dinucleotides and a propensity for G|C-rich sequence elements to conform energetically favourably at most locations around the histone octamer, which rationalizes G|C% as the most predictive factor for nucleosome occupancy in vivo. In addition, our findings reveal dominant constraints on double helix conformation by H3-H4 relative to H2A-H2B binding and DNA sequence context-dependency underlying nucleosome structure, positioning and stability. This provides a basis for improved prediction of nucleosomal properties and the design of tailored DNA constructs for chromatin investigations.

PubMed: 22453276
DOI: 10.1093/nar/gks261

実験手法

X-RAY DIFFRACTION (2.2 Å)