3USQ

Structure of D159S/Y194F glycogenin mutant truncated at residue 270

3USQ の概要

• エントリーDOI: 10.2210/pdb3usq/pdb
• 関連するPDBエントリー: 1LL0 1LL2 1LL3 1ZCT 1ZCU 1ZCV 1ZCY 3UNS
• 分子名称: Glycogenin-1, GLYCEROL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: glucosyltransferase, transferase
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32783.30

構造登録者

Issoglio, F.M.,Carrizo, M.E.,Romero, J.M.,Curtino, J.A. (登録日: 2011-11-23, 公開日: 2011-12-14, 最終更新日: 2024-02-28)

主引用文献

Issoglio, F.M.,Carrizo, M.E.,Romero, J.M.,Curtino, J.A.
Mechanisms of monomeric and dimeric glycogenin autoglucosylation.
J.Biol.Chem., 287:1955-1961, 2012

PubMed Abstract: Initiation of glucose polymerization by glycogenin autoglucosylation at Tyr-194 is required to prime de novo biosynthesis of glycogen. It has been proposed that the synthesis of the primer proceeds by intersubunit glucosylation of dimeric glycogenin, even though it has not been demonstrated that this mechanism is responsible for the described polymerization extent of 12 glucoses produced by the dimer. We reported previously the intramonomer glucosylation capability of glycogenin without determining the extent of autoglucopolymerization. Here, we show that the maximum specific autoglucosylation extent (MSAE) produced by the non-glucosylated glycogenin monomer is 13.3 ± 1.9 glucose units, similar to the 12.5 ± 1.4 glucose units measured for the dimer. The mechanism and capacity of the dimeric enzyme to carry out full glucopolymerization were also evaluated by construction of heterodimers able to glucosylate exclusively by intrasubunit or intersubunit reaction mechanisms. The MSAE of non-glucosylated glycogenin produced by dimer intrasubunit glucosylation was 16% of that produced by the monomer. However, partially glucosylated glycogenin was able to almost complete its autoglucosylation by the dimer intrasubunit mechanism. The MSAE produced by heterodimer intersubunit glucosylation was 60% of that produced by the wild-type dimer. We conclude that both intrasubunit and intersubunit reaction mechanisms are necessary for the dimeric enzyme to acquire maximum autoglucosylation. The full glucopolymerization capacity of monomeric glycogenin indicates that the enzyme is able to synthesize the glycogen primer without the need for prior dimerization.

PubMed: 22128147
DOI: 10.1074/jbc.M111.287813

実験手法

X-RAY DIFFRACTION (2.4 Å)