3US0

Structure of p63 DNA Binding Domain in Complex with a 22 Base Pair A/T Rich Response Element Containing a Two Base Pair "AT" Spacer Between Half Sites

3US0 の概要

• エントリーDOI: 10.2210/pdb3us0/pdb
• 関連するPDBエントリー: 3QYM 3QYN 3US1 3US2
• 分子名称: Tumor protein 63, 5'-D(*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*T)-3', ZINC ION, ... (4 entities in total)
• 機能のキーワード: b-dna double helix, zinc binding, beta sandwich, greek key, transcription factor, nucleus, transcription activator-dna complex, transcription activator/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus : Q9H3D4
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 104493.91

構造登録者

Chen, C.,Herzberg, O. (登録日: 2011-11-22, 公開日: 2012-02-01, 最終更新日: 2023-09-13)

主引用文献

Chen, C.,Gorlatova, N.,Herzberg, O.
Pliable DNA Conformation of Response Elements Bound to Transcription Factor p63.
J.Biol.Chem., 287:7477-7486, 2012

PubMed Abstract: We show that changes in the nucleotide sequence alter the DNA conformation in the crystal structures of p63 DNA-binding domain (p63DBD) bound to its response element. The conformation of a 22-bp canonical response element containing an AT spacer between the two half-sites is unaltered compared with that containing a TA spacer, exhibiting superhelical trajectory. In contrast, a GC spacers abolishes the DNA superhelical trajectory and exhibits less bent DNA, suggesting that increased GC content accompanies increased double helix rigidity. A 19-bp DNA, representing an AT-rich response element with overlapping half-sites, maintains superhelical trajectory and reveals two interacting p63DBD dimers crossing one another at 120°. p63DBD binding assays to response elements of increasing length complement the structural studies. We propose that DNA deformation may affect promoter activity, that the ability of p63DBD to bind to superhelical DNA suggests that it is capable of binding to nucleosomes, and that overlapping response elements may provide a mechanism to distinguish between p63 and p53 promoters.

PubMed: 22247550
DOI: 10.1074/jbc.M111.315820

実験手法

X-RAY DIFFRACTION (2.5 Å)