3UON

Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist

3UON の概要

• エントリーDOI: 10.2210/pdb3uon/pdb
• 分子名称: Human M2 muscarinic acetylcholine, receptor T4 lysozyme fusion protein, (3R)-1-azabicyclo[2.2.2]oct-3-yl hydroxy(diphenyl)acetate, beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: g protein-coupled receptor, gpcr, acetylcholine receptor, signaling protein-antagonist complex, signaling protein/antagonist
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53283.02

構造登録者

Haga, K.,Kruse, A.C.,Asada, H.,Yurugi-Kobayashi, T.,Shiroishi, M.,Zhang, C.,Weis, W.I.,Okada, T.,Kobilka, B.K.,Haga, T.,Kobayashi, T. (登録日: 2011-11-16, 公開日: 2012-02-01, 最終更新日: 2023-09-13)

主引用文献

Haga, K.,Kruse, A.C.,Asada, H.,Yurugi-Kobayashi, T.,Shiroishi, M.,Zhang, C.,Weis, W.I.,Okada, T.,Kobilka, B.K.,Haga, T.,Kobayashi, T.
Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist.
Nature, 482:547-551, 2012

PubMed Abstract: The parasympathetic branch of the autonomic nervous system regulates the activity of multiple organ systems. Muscarinic receptors are G-protein-coupled receptors that mediate the response to acetylcholine released from parasympathetic nerves. Their role in the unconscious regulation of organ and central nervous system function makes them potential therapeutic targets for a broad spectrum of diseases. The M2 muscarinic acetylcholine receptor (M2 receptor) is essential for the physiological control of cardiovascular function through activation of G-protein-coupled inwardly rectifying potassium channels, and is of particular interest because of its extensive pharmacological characterization with both orthosteric and allosteric ligands. Here we report the structure of the antagonist-bound human M2 receptor, the first human acetylcholine receptor to be characterized structurally, to our knowledge. The antagonist 3-quinuclidinyl-benzilate binds in the middle of a long aqueous channel extending approximately two-thirds through the membrane. The orthosteric binding pocket is formed by amino acids that are identical in all five muscarinic receptor subtypes, and shares structural homology with other functionally unrelated acetylcholine binding proteins from different species. A layer of tyrosine residues forms an aromatic cap restricting dissociation of the bound ligand. A binding site for allosteric ligands has been mapped to residues at the entrance to the binding pocket near this aromatic cap. The structure of the M2 receptor provides insights into the challenges of developing subtype-selective ligands for muscarinic receptors and their propensity for allosteric regulation.

PubMed: 22278061
DOI: 10.1038/nature10753

実験手法

X-RAY DIFFRACTION (3 Å)