3ULR

Lysozyme contamination facilitates crystallization of a hetero-trimericCortactin:Arg:Lysozyme complex

3ULR の概要

• エントリーDOI: 10.2210/pdb3ulr/pdb
• 分子名称: Lysozyme C, Src substrate cortactin, Abelson tyrosine-protein kinase 2, ... (4 entities in total)
• 機能のキーワード: sh3, protein-protein interaction, hydrolase, protein binding
• 由来する生物種: Gallus gallus (bantam,chickens); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton: P00698 Q60598; Secreted: P42684
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 23403.33

構造登録者

Liu, W.,MacGrath, S.,Koleske, A.J.,Boggon, T.J. (登録日: 2011-11-11, 公開日: 2012-01-11, 最終更新日: 2024-11-27)

主引用文献

Liu, W.,Macgrath, S.M.,Koleske, A.J.,Boggon, T.J.
Lysozyme contamination facilitates crystallization of a heterotrimeric cortactin-Arg-lysozyme complex.
Acta Crystallogr.,Sect.F, 68:154-158, 2012

PubMed Abstract: Crystallization of contaminating proteins is a frequently encountered problem for macromolecular crystallographers. In this study, an attempt was made to obtain a binary cocrystal structure of the SH3 domain of cortactin and a 17-residue peptide from the Arg nonreceptor tyrosine kinase encompassing a PxxPxxPxxP (PxxP1) motif. However, cocrystals could only be obtained in the presence of trace amounts of a contaminating protein. A structure solution obtained by molecular replacement followed by ARP/wARP automatic model building allowed a 'sequence-by-crystallography' approach to discover that the contaminating protein was lysozyme. This 1.65 Å resolution crystal structure determination of a 1:1:1 heterotrimeric complex of Arg, cortactin and lysozyme thus provides an unusual `caveat emptor' warning of the dangers that underpurified proteins harbor for macromolecular crystallographers.

PubMed: 22297987
DOI: 10.1107/S1744309111056132

実験手法

X-RAY DIFFRACTION (1.65 Å)