3ULL

HUMAN MITOCHONDRIAL SINGLE-STRANDED DNA BINDING PROTEIN

Summary for 3ULL

• Entry DOI: 10.2210/pdb3ull/pdb
• Descriptor: DNA BINDING PROTEIN (2 entities in total)
• Functional Keywords: dna-binding protein, dna replication, mitochondrion, transit peptide, dna binding protein
• Biological source: Homo sapiens (human)
• Cellular location: Mitochondrion: Q04837
• Total number of polymer chains: 2
• Total formula weight: 30432.25

Authors

Yang, C.,Curth, U.,Urbanke, C.,Kang, C. (deposition date: 1996-12-11, release date: 1997-10-15, Last modification date: 2024-02-28)

Primary citation

Yang, C.,Curth, U.,Urbanke, C.,Kang, C.
Crystal structure of human mitochondrial single-stranded DNA binding protein at 2.4 A resolution.
Nat.Struct.Biol., 4:153-157, 1997

PubMed Abstract: We solved the crystal structure of the homotetrameric single-stranded DNA binding (SSB) protein from human mitochondria at a resolution of 2.4 A. The tetramer is formed by two dimers interacting head-to-head and shows D2 symmetry. Sequence-related tetrameric SSB proteins occur in prokaryotes and eukaryotic mitochondria; this is the first report of an atomic resolution structure of this type of protein. Using biochemical data and analysis of sequence homologies, we were able to correlate the functional properties with structure. We propose that ssDNA wraps around the tetrameric HsmtSSB protein through electropositive channels guided by flexible loops.

PubMed: 9033597
DOI: 10.1038/nsb0297-153

Experimental method

X-RAY DIFFRACTION (2.4 Å)