Summary for 3UJ3
| Entry DOI | 10.2210/pdb3uj3/pdb |
| Related | 1ZR2 1ZR4 3PKZ 3PLO |
| Descriptor | DNA-invertase (1 entity in total) |
| Functional Keywords | helix-turn-helix, site-specific recombinase, recombination |
| Biological source | Enterobacteria phage Mu |
| Total number of polymer chains | 1 |
| Total formula weight | 21756.28 |
| Authors | Ritacco, C.J.,Wang, J.,Kamtekar, S.,Steitz, T.A. (deposition date: 2011-11-07, release date: 2012-12-05, Last modification date: 2023-09-13) |
| Primary citation | Ritacco, C.J.,Kamtekar, S.,Wang, J.,Steitz, T.A. Crystal structure of an intermediate of rotating dimers within the synaptic tetramer of the G-segment invertase. Nucleic Acids Res., 41:2673-2682, 2013 Cited by PubMed Abstract: The serine family of site-specific DNA recombination enzymes accomplishes strand cleavage, exchange and religation using a synaptic protein tetramer. A double-strand break intermediate in which each protein subunit is covalently linked to the target DNA substrate ensures that the recombination event will not damage the DNA. The previous structure of a tetrameric synaptic complex of γδ resolvase linked to two cleaved DNA strands had suggested a rotational mechanism of recombination in which one dimer rotates 180° about the flat exchange interface for strand exchange. Here, we report the crystal structure of a synaptic tetramer of an unliganded activated mutant (M114V) of the G-segment invertase (Gin) in which one dimer half is rotated by 26° or 154° relative to the other dimer when compared with the dimers in the synaptic complex of γδ resolvase. Modeling shows that this rotational orientation of Gin is not compatible with its being able to bind uncleaved DNA, implying that this structure represents an intermediate in the process of strand exchange. Thus, our structure provides direct evidence for the proposed rotational mechanism of site-specific recombination. PubMed: 23275567DOI: 10.1093/nar/gks1303 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.51 Å) |
Structure validation
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