3UIY

Crystal structure of SefD_dscA in H2O

Summary for 3UIY

Entry DOI	10.2210/pdb3uiy/pdb
Related	3UIZ
Descriptor	Chimera protein of SefD and SefA (1 entity in total)
Functional Keywords	deuterium, pilin, immunoglobulin, immunoglobulin like fold, chaperone-usher minor pilin domain, extracellular membrane surface, structural protein
Biological source	Salmonella enterica subsp. enterica serovar Enteritidis More
Cellular location	Fimbrium: P12061
Total number of polymer chains	1
Total formula weight	16914.17
Authors	Garnett, J.A.,Wei-chao, L.,Liu, B.,Matthews, S.J. (deposition date: 2011-11-07, release date: 2012-05-02, Last modification date: 2023-09-13)
Primary citation	Liu, B.,Garnett, J.A.,Lee, W.C.,Lin, J.,Salgado, P.,Taylor, J.,Xu, Y.,Lambert, S.,Cota, E.,Matthews, S. Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide. Biochem.Biophys.Res.Commun., 421:208-213, 2012 Cited by PubMed Abstract: The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD. PubMed: 22497887 DOI: 10.1016/j.bbrc.2012.03.136 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.1 Å)

Structure validation