3UIY

Crystal structure of SefD_dscA in H2O

3UIY の概要

• エントリーDOI: 10.2210/pdb3uiy/pdb
• 関連するPDBエントリー: 3UIZ
• 分子名称: Chimera protein of SefD and SefA (1 entity in total)
• 機能のキーワード: deuterium, pilin, immunoglobulin, immunoglobulin like fold, chaperone-usher minor pilin domain, extracellular membrane surface, structural protein
• 由来する生物種: Salmonella enterica subsp. enterica serovar Enteritidis; 詳細
• 細胞内の位置: Fimbrium: P12061
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16914.17

構造登録者

Garnett, J.A.,Wei-chao, L.,Liu, B.,Matthews, S.J. (登録日: 2011-11-07, 公開日: 2012-05-02, 最終更新日: 2023-09-13)

主引用文献

Liu, B.,Garnett, J.A.,Lee, W.C.,Lin, J.,Salgado, P.,Taylor, J.,Xu, Y.,Lambert, S.,Cota, E.,Matthews, S.
Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide.
Biochem.Biophys.Res.Commun., 421:208-213, 2012

PubMed Abstract: The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.

PubMed: 22497887
DOI: 10.1016/j.bbrc.2012.03.136

実験手法

X-RAY DIFFRACTION (3.1 Å)