3UE5
ECP-cleaved Actin in complex with Spir domain D
3UE5 の概要
エントリーDOI | 10.2210/pdb3ue5/pdb |
分子名称 | Actin, alpha skeletal muscle, Protein spire, CALCIUM ION, ... (6 entities in total) |
機能のキーワード | contractile protein, contractile protein-transport protein complex, contractile protein/transport protein |
由来する生物種 | Drosophila melanogaster (Fruit fly) 詳細 |
細胞内の位置 | Cytoplasm, cytoskeleton: P68135 Q9U1K1 |
タンパク質・核酸の鎖数 | 2 |
化学式量合計 | 49801.36 |
構造登録者 | Chen, C.,Phillips, M.,Sawaya, M.R.,Ralston, C.Y.,Quinlan, M.E. (登録日: 2011-10-28, 公開日: 2012-02-15, 最終更新日: 2024-11-27) |
主引用文献 | Chen, C.K.,Sawaya, M.R.,Phillips, M.L.,Reisler, E.,Quinlan, M.E. Multiple Forms of Spire-Actin Complexes and their Functional Consequences. J.Biol.Chem., 287:10684-10692, 2012 Cited by PubMed Abstract: Spire is a WH2 domain-containing actin nucleator essential for establishing an actin mesh during oogenesis. In vitro, in addition to nucleating filaments, Spire can sever them and sequester actin monomers. Understanding how Spire is capable of these disparate functions and which are physiologically relevant is an important goal. To study severing, we examined the effect of Drosophila Spire on preformed filaments in bulk and single filament assays. We observed rapid depolymerization of actin filaments by Spire, which we conclude is largely due to its sequestration activity and enhanced by its weak severing activity. We also studied the solution and crystal structures of Spire-actin complexes. We find structural and functional differences between constructs containing four WH2 domains (Spir-ABCD) and two WH2 domains (Spir-CD) that may provide insight into the mechanisms of nucleation and sequestration. Intriguingly, we observed lateral interactions between actin monomers associated with Spir-ABCD, suggesting that the structures built by these four tandem WH2 domains are more complex than originally imagined. Finally, we propose that Spire-actin mixtures contain both nuclei and sequestration structures. PubMed: 22334675DOI: 10.1074/jbc.M111.317792 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.76 Å) |
構造検証レポート
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