3UD7

Crystal Structure Analysis of FGF1-Disaccharide(NI21) complexes

3UD7 の概要

• エントリーDOI: 10.2210/pdb3ud7/pdb
• 関連するPDBエントリー: 3UD8 3UD9 3UDA
• 分子名称: Heparin-binding growth factor 1, 2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-1-O-methyl-2-O-sulfo-alpha-L-idopyranuronic acid, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: heparin/heparan sulfate binding, hormone
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 49066.46

構造登録者

Hung, S.-C.,Shi, Z. (登録日: 2011-10-27, 公開日: 2012-11-28, 最終更新日: 2023-11-01)

主引用文献

Hu, Y.-P.,Zhong, Y.-Q.,Chen, Z.-G.,Chen, C.-Y.,Shi, Z.,Zulueta, M.M.L.,Ku, C.-C.,Lee, P.-Y.,Wang, C.-C.,Hung, S.-C.
Divergent synthesis of 48 heparan sulfate-based disaccharides and probing the specific sugar-fibroblast growth factor-1 interaction
J.Am.Chem.Soc., 134:20722-20727, 2012

PubMed Abstract: Several biological processes involve glycans, yet understanding their ligand specificities is impeded by their inherent diversity and difficult acquisition. Generating broad synthetic sugar libraries for bioevaluations is a powerful tool in unraveling glycan structural information. In the case of the widely distributed heparan sulfate (HS), however, the 48 theoretical possibilities for its repeating disaccharide call for synthetic approaches that should minimize the effort in an undoubtedly huge undertaking. Here we employed a divergent strategy to afford all 48 HS-based disaccharides from just two orthogonally protected disaccharide precursors. Different combinations and sequence of transformation steps were applied with many downstream intermediates leading up to multiple target products. With the full disaccharide library in hand, affinity screening with fibroblast growth factor-1 (FGF-1) revealed that four of the synthetic sugars bind to FGF-1. The molecular details of the interaction were further clarified through X-ray analysis of the sugar-protein cocrystals. The capability of comprehensive sugar libraries in providing key insights in glycan-ligand interaction is, thus, highlighted.

PubMed: 23240683
DOI: 10.1021/ja3090065

実験手法

X-RAY DIFFRACTION (2.8 Å)