3UBC

Oxygen-bound hell's gate globin I by LB nanotemplate method

3UBC の概要

• エントリーDOI: 10.2210/pdb3ubc/pdb
• 関連するPDBエントリー: 3UBV
• 分子名称: Hemoglobin-like flavoprotein, PROTOPORPHYRIN IX CONTAINING FE, OXYGEN MOLECULE, ... (4 entities in total)
• 機能のキーワード: oxygen-bound, autoxidation, nanotemplate, langmuir-blodgett, thin films, oxygen transport
• 由来する生物種: Methylacidiphilum infernorum V4
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 46456.63

構造登録者

Belmonte, L.,Scudieri, D.,Nicolini, C.,Pechkova, E. (登録日: 2011-10-24, 公開日: 2012-03-28, 最終更新日: 2024-02-28)

主引用文献

Pechkova, E.,Scudieri, D.,Belmonte, L.,Nicolini, C.
Oxygen-bound Hell's gate globin I by classical versus LB nanotemplate method.
J.Cell.Biochem., 8:793-794, 2012

PubMed Abstract: X-ray atomic structure of recombinant Hell's gate globin I (HGbI) from Methylacidophilum infernorum was calculated from the X-ray diffraction data of two different types of crystals: obtained by classical hanging drop and by LB nanotemplate method under the same crystallization conditions. After the accurate comparison of crystallographic parameters and electron density maps of two structures they appears to be quite similar, while the quality of the crystals grown by LB nanotemplate method was higher then of those grown by classical method. Indeed, the resolution of the LB crystal structure was 1.65 Å, while classical crystals showed only 3.2 Å resolution. Moreover, the reproducibility of this result in the case of LB crystals was much better-nine crystals from 10 gave the same structural results, while only two of 10 classical crystals were appropriate for the X-ray structure resolution.

PubMed: 22415930
DOI: 10.1002/jcb.24131

実験手法

X-RAY DIFFRACTION (1.65 Å)