3U35

Crystal structure of the general stress FMN/FAD binding protein from the phytopathogen Xanthomonas citri

3U35 の概要

• エントリーDOI: 10.2210/pdb3u35/pdb
• 関連するPDBエントリー: 1FLM 2I02 2OU5 3DMB 3U34
• 分子名称: General stress protein, TRIETHYLENE GLYCOL (3 entities in total)
• 機能のキーワード: xanthomonas citri general stress protein fmn binding protein fad binding protein, pnp-oxidase like fold, fmn/fad, protein binding
• 由来する生物種: Xanthomonas axonopodis pv. citri (Citrus canker)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 80735.98

構造登録者

Hilario, E.,Li, Y.,Fan, L. (登録日: 2011-10-04, 公開日: 2012-06-27, 最終更新日: 2024-02-28)

主引用文献

Hilario, E.,Li, Y.,Niks, D.,Fan, L.
The structure of a Xanthomonas general stress protein involved in citrus canker reveals its flavin-binding property.
Acta Crystallogr.,Sect.D, 68:846-853, 2012

PubMed Abstract: Xanthomonas citri pv. citri (Xac) causes citrus canker and affects citrus agriculture worldwide. Functional genetic analysis has indicated that a putative general stress protein (XacGSP) encoded by the Xac2369 gene is involved in the bacterial infection. In this report, the crystal structure of XacGSP was determined to 2.5 Å resolution. There are four XacGSP molecules in the crystal asymmetric unit. Each XacGSP monomer folds into a six-stranded antiparallel β-barrel flanked by five α-helices. A C-terminal extension protrudes from the sixth β-strand of the β-barrel and pairs with its counterpart from another monomer to form a bridge between the two subunits of an XacGSP dimer. Two XacGSP dimers cross over each other to form a tetramer; the β-barrels from one dimer contact the β-barrels of the other, while the two bridges are distant from each other and do not make contacts. The three-dimensional structure of the XacGSP monomer is very similar to those of pyridoxine 5-phosphate oxidases, a group of enzymes that use flavin mononucleotide (FMN) as a cofactor. Consistent with this, purified XacGSP protein binds to both FMN and flavin adenine dinucleotide (FAD), suggesting that XacGSP may help the bacteria to react against the oxidative stress induced by the defense mechanisms of the plant.

PubMed: 22751670
DOI: 10.1107/S0907444912014126

実験手法

X-RAY DIFFRACTION (2.5 Å)