3U0D

The structure of human Siderocalin bound to the bacterial siderophore 2,3-DHBA

3U0D の概要

• エントリーDOI: 10.2210/pdb3u0d/pdb
• 分子名称: Neutrophil gelatinase-associated lipocalin, FE (III) ION, 2,3-DIHYDROXY-BENZOIC ACID, ... (5 entities in total)
• 機能のキーワード: siderophore, beta-barrel, structural genomics, seattle structural genomics center for infectious disease, ssgcid, transport protein, antimicrobial protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted : P80188
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 92571.03

構造登録者

Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2011-09-28, 公開日: 2011-10-12, 最終更新日: 2024-11-06)

主引用文献

Correnti, C.,Richardson, V.,Sia, A.K.,Bandaranayake, A.D.,Ruiz, M.,Suryo Rahmanto, Y.,Kovacevic, Z.,Clifton, M.C.,Holmes, M.A.,Kaiser, B.K.,Barasch, J.,Raymond, K.N.,Richardson, D.R.,Strong, R.K.
Siderocalin/Lcn2/NGAL/24p3 does not drive apoptosis through gentisic acid mediated iron withdrawal in hematopoietic cell lines.
Plos One, 7:e43696-e43696, 2012

PubMed Abstract: Siderocalin (also lipocalin 2, NGAL or 24p3) binds iron as complexes with specific siderophores, which are low molecular weight, ferric ion-specific chelators. In innate immunity, siderocalin slows the growth of infecting bacteria by sequestering bacterial ferric siderophores. Siderocalin also binds simple catechols, which can serve as siderophores in the damaged urinary tract. Siderocalin has also been proposed to alter cellular iron trafficking, for instance, driving apoptosis through iron efflux via BOCT. An endogenous siderophore composed of gentisic acid (2,5-dihydroxybenzoic acid) substituents was proposed to mediate cellular efflux. However, binding studies reported herein contradict the proposal that gentisic acid forms high-affinity ternary complexes with siderocalin and iron, or that gentisic acid can serve as an endogenous siderophore at neutral pH. We also demonstrate that siderocalin does not induce cellular iron efflux or stimulate apoptosis, questioning the role siderocalin plays in modulating iron metabolism.

PubMed: 22928018
DOI: 10.1371/journal.pone.0043696

実験手法

X-RAY DIFFRACTION (2.51 Å)