3TWC

Crystal structure of broad and potent HIV-1 neutralizing antibody PGT127 in complex with Man9

3TWC の概要

• エントリーDOI: 10.2210/pdb3twc/pdb
• 関連するPDBエントリー: 3TV3 3TYG
• 分子名称: PGT127 light chain, Ig lambda-2 chain C regions, PGT127 heavy chain, Ig gamma-1 chain C region, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose, ... (5 entities in total)
• 機能のキーワード: fab, hiv-1 neutralizing antibody, gp120, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49452.81

構造登録者

Pejchal, R.,Wilson, I.A. (登録日: 2011-09-21, 公開日: 2011-10-19, 最終更新日: 2024-10-16)

主引用文献

Pejchal, R.,Doores, K.J.,Walker, L.M.,Khayat, R.,Huang, P.S.,Wang, S.K.,Stanfield, R.L.,Julien, J.P.,Ramos, A.,Crispin, M.,Depetris, R.,Katpally, U.,Marozsan, A.,Cupo, A.,Maloveste, S.,Liu, Y.,McBride, R.,Ito, Y.,Sanders, R.W.,Ogohara, C.,Paulson, J.C.,Feizi, T.,Scanlan, C.N.,Wong, C.H.,Moore, J.P.,Olson, W.C.,Ward, A.B.,Poignard, P.,Schief, W.R.,Burton, D.R.,Wilson, I.A.
A potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield.
Science, 334:1097-1103, 2011

PubMed Abstract: The HIV envelope (Env) protein gp120 is protected from antibody recognition by a dense glycan shield. However, several of the recently identified PGT broadly neutralizing antibodies appear to interact directly with the HIV glycan coat. Crystal structures of antigen-binding fragments (Fabs) PGT 127 and 128 with Man(9) at 1.65 and 1.29 angstrom resolution, respectively, and glycan binding data delineate a specific high mannose-binding site. Fab PGT 128 complexed with a fully glycosylated gp120 outer domain at 3.25 angstroms reveals that the antibody penetrates the glycan shield and recognizes two conserved glycans as well as a short β-strand segment of the gp120 V3 loop, accounting for its high binding affinity and broad specificity. Furthermore, our data suggest that the high neutralization potency of PGT 127 and 128 immunoglobulin Gs may be mediated by cross-linking Env trimers on the viral surface.

PubMed: 21998254
DOI: 10.1126/science.1213256

実験手法

X-RAY DIFFRACTION (1.65 Å)