3TSR

X-ray structure of mouse ribonuclease inhibitor complexed with mouse ribonuclease 1

3TSR の概要

• エントリーDOI: 10.2210/pdb3tsr/pdb
• 分子名称: Ribonuclease pancreatic, Ribonuclease inhibitor, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: hydrolase, hydrolase inhibitor, leucine-rich repeat, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Secreted: P00683; Cytoplasm : Q91VI7
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 257559.07

構造登録者

Chang, A.,Lomax, J.E.,Bingman, C.A.,Raines, R.T.,Phillips Jr., G.N. (登録日: 2011-09-13, 公開日: 2012-09-19, 最終更新日: 2024-10-16)

主引用文献

Lomax, J.E.,Bianchetti, C.M.,Chang, A.,Phillips, G.N.,Fox, B.G.,Raines, R.T.
Functional evolution of ribonuclease inhibitor: insights from birds and reptiles.
J.Mol.Biol., 426:3041-3056, 2014

PubMed Abstract: Ribonuclease inhibitor (RI) is a conserved protein of the mammalian cytosol. RI binds with high affinity to diverse secretory ribonucleases (RNases) and inhibits their enzymatic activity. Although secretory RNases are found in all vertebrates, the existence of a non-mammalian RI has been uncertain. Here, we report on the identification and characterization of RI homologs from chicken and anole lizard. These proteins bind to RNases from multiple species but exhibit much greater affinity for their cognate RNases than for mammalian RNases. To reveal the basis for this differential affinity, we determined the crystal structure of mouse, bovine, and chicken RI·RNase complexes to a resolution of 2.20, 2.21, and 1.92Å, respectively. A combination of structural, computational, and bioinformatic analyses enabled the identification of two residues that appear to contribute to the differential affinity for RNases. We also found marked differences in oxidative instability between mammalian and non-mammalian RIs, indicating evolution toward greater oxygen sensitivity in RIs from mammalian species. Taken together, our results illuminate the structural and functional evolution of RI, along with its dynamic role in vertebrate biology.

PubMed: 24941155
DOI: 10.1016/j.jmb.2014.06.007

実験手法

X-RAY DIFFRACTION (2.1999 Å)