3TNF

LidA from Legionella in complex with active Rab8a

3TNF の概要

• エントリーDOI: 10.2210/pdb3tnf/pdb
• 関連するPDBエントリー: 3QBT
• 分子名称: Ras-related protein Rab-8A, LidA, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: protein transport, vesicular trafficking, gtpase, legionella pneumophila, rab8a, vesicle recuitment, lcv, drra, sidm, rab-effector, vesicular transport, gdp/gtp binding rab-binding, er, golgi, plasmamembrane
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential): P61006
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 65668.59

構造登録者

Schoebel, S.,Cichy, A.L.,Goody, R.S.,Itzen, A. (登録日: 2011-09-01, 公開日: 2011-11-02, 最終更新日: 2024-02-28)

主引用文献

Schoebel, S.,Cichy, A.L.,Goody, R.S.,Itzen, A.
Protein LidA from Legionella is a Rab GTPase supereffector.
Proc.Natl.Acad.Sci.USA, 108:17945-17950, 2011

PubMed Abstract: The causative agent of Legionnaires disease, Legionella pneumophila, injects several hundred proteins into the cell it infects, many of which interfere with or exploit vesicular transport processes. One of these proteins, LidA, has been described as a Rab effector (i.e., a molecule that interacts preferentially with the GTP-bound form of Rab). We describe here the structure and biochemistry of a complex between the Rab-binding domain of LidA and active Rab8a. LidA displays structural peculiarities in binding to Rab8a, forming a considerably extended interface in comparison to known mammalian Rab effectors, and involving regions of the GTPase that are not seen in other Rab:effector complexes. In keeping with this extended binding interface, which involves four α-helices and two pillar-like structures of LidA, the stability of LidA-Rab interactions is dramatically greater than for other such complexes. For Rab1b and Rab8a, these affinities are extraordinarily high, but for the more weakly bound Rab6a, K(d) values of 4 nM for the inactive and 30 pM for the active form were found. Rab1b and Rab8a appear to bind LidA with K(d) values in the low picomolar range, making LidA a Rab supereffector.

PubMed: 22011575
DOI: 10.1073/pnas.1113133108

実験手法

X-RAY DIFFRACTION (2.5 Å)