3TMV

X-Ray Radiation Damage to HEWL Crystals soaked in 100mM Sodium Nitrate (Dose=0.12MGy)

3TMV の概要

• エントリーDOI: 10.2210/pdb3tmv/pdb
• 関連するPDBエントリー: 3TMU 3TMW 3TMX
• 分子名称: Lysozyme C, CHLORIDE ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Gallus gallus (bantam,chickens)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16564.27

構造登録者

Kmetko, J.,Warkentin, M.A.,Englich, U.,Thorne, R.E. (登録日: 2011-08-31, 公開日: 2012-08-22, 最終更新日: 2024-10-16)

主引用文献

Kmetko, J.,Warkentin, M.,Englich, U.,Thorne, R.E.
Can radiation damage to protein crystals be reduced using small-molecule compounds?
Acta Crystallogr.,Sect.D, 67:881-893, 2011

PubMed Abstract: Recent studies have defined a data-collection protocol and a metric that provide a robust measure of global radiation damage to protein crystals. Using this protocol and metric, 19 small-molecule compounds (introduced either by cocrystallization or soaking) were evaluated for their ability to protect lysozyme crystals from radiation damage. The compounds were selected based upon their ability to interact with radiolytic products (e.g. hydrated electrons, hydrogen, hydroxyl and perhydroxyl radicals) and/or their efficacy in protecting biological molecules from radiation damage in dilute aqueous solutions. At room temperature, 12 compounds had no effect and six had a sensitizing effect on global damage. Only one compound, sodium nitrate, appeared to extend crystal lifetimes, but not in all proteins and only by a factor of two or less. No compound provided protection at T=100 K. Scavengers are ineffective in protecting protein crystals from global damage because a large fraction of primary X-ray-induced excitations are generated in and/or directly attack the protein and because the ratio of scavenger molecules to protein molecules is too small to provide appreciable competitive protection. The same reactivity that makes some scavengers effective radioprotectors in protein solutions may explain their sensitizing effect in the protein-dense environment of a crystal. A more productive focus for future efforts may be to identify and eliminate sensitizing compounds from crystallization solutions.

PubMed: 21931220
DOI: 10.1107/S0907444911032835

実験手法

X-RAY DIFFRACTION (1.9 Å)